| Primary Identifier | MGI:2683406 | Allele Type | Targeted |
| Attribute String | Hypomorph | Gene | Pparg |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A hypomorphic allele was generated by inserting an frt flanked neomycin selection cassette in the intron following exon B. The targeting vector also included a proline to alaline missense mutation at codon 12 (P12A) and loxP sites flanking exon B and the neo cassette. mRNA corresponding to both alternatively spliced transcripts 1 and 2 were significantly reduced in homozygous mutant white adipose tissue. While variant 2 transcript was nearly undetected in homozygous mutant brown adipose tissue, Variant 1 was significantly increased relative to wild-type. |
