| Primary Identifier | MGI:6455404 | Allele Type | Transgenic |
| Attribute String | Reporter | Gene | Tg(CAG-mRuby2*,-mClover*)1Srgt |
| Strain of Origin | (C57BL/6 x SJL)F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/cas9 endonuclease-mediated genome editing was used to introduce a CMV enhancer/chicken beta-actin (CAG) promoter followed by a loxP-flanked STOP cassette, an H2B/mRuby2 fusion protein, a P2A self-cleaving peptide sequence, and an ERK-KTR/mClover fusion protein into the Gt(ROSA)26Sor locus. ERK-KTR consists of a docking site (a fragment of human ELK1, an ERK substrate), nuclear localization (NLS) and export (NES) signals, and mClover green/yellow fluorescent protein. Through nanopore sequencing and PCR verification, it was determined that the vector, along with the Rosa26 homology arms, randomly inserted into a noncoding region of chromosome 13. |
