Primary Identifier | MGI:5297083 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Bhmt |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | One loxAPA site was inserted in intron 5 240 bp upstream of exon 6 and an FRT flanked MC1-neomycin cassette followed by a second loxP site was placed 330 bp from exon 7. The neomycin selection cassette was excised by crossing to Tg(ACTFLPe)9205Dym mice. Exons 6 and 7, containing the essential zinc binding domain, were then removed by crossing to Tg(EIIa-cre)C5379Lmgd mice. |