| Primary Identifier | MGI:6810181 | Allele Type | Targeted |
| Attribute String | Epitope tag | Gene | Themis |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Sequence for a OneâSTrEPâtag (OST) and a Gly-Ser-Gly spacer was inserted in-frame at the 5â² end of the gene coding sequence. The loxP site flanked mouse Ace (angiotensin I converting enzyme (peptidyl-dipeptidase A) 1) promoter, cre recombinase gene, and neomycin resistance gene cassette sequence that was also inserted, was removed through Cre-mediated self-excision in the male germline. The fusion of the OST to the protein can be used in affinity purification. |
