| First Author | Liu D | Year | 2024 |
| Journal | Nat Immunol | Volume | 25 |
| Issue | 1 | Pages | 142-154 |
| PubMed ID | 38049580 | Mgi Jnum | J:346729 |
| Mgi Id | MGI:7617164 | Doi | 10.1038/s41590-023-01690-z |
| Citation | Liu D, et al. (2024) Dynamic encounters with red blood cells trigger splenic marginal zone B cell retention and function. Nat Immunol 25(1):142-154 |
| abstractText | Spleen marginal zone (MZ) B cells are important for antibody responses against blood-borne antigens. The signals they use to detect exposure to blood are not well defined. Here, using intravital two-photon microscopy in mice, we observe transient contacts between MZ B cells and red blood cells that are in flow. We show that MZ B cells use adhesion G-protein-coupled receptor ADGRE5 (CD97) for retention in the spleen. CD97 function in MZ B cells depends on its ability to undergo autoproteolytic cleavage and signaling via Galpha(13) and ARHGEF1. Red blood cell expression of the CD97 ligand CD55 is required for MZ B cell homeostasis. Applying a pulling force on CD97-transfected cells using an optical C-trap and CD55(+) beads leads to accumulation of active RhoA and membrane retraction. Finally, we show that CD97 deficiency leads to a reduced T cell-independent IgM response. Thus, our studies provide evidence that MZ B cells use mechanosensing to position in a manner that enhances antibody responses against blood-borne antigens. |
