| Primary Identifier | MGI:7624348 | Allele Type | Targeted |
| Gene | Nphs1 | Transmission | Germline |
| Strain of Origin | C57BL/6J x 129S6/SvEvTac | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Tyrosine codons 1191 (TAT) and 1208 (TAT) in exon 28 and 1232 (TAC) in exon 29 were changed to phenylalanine (TTT, TTT, TTC) (p.Y1191F, p.Y1208F, p.Y1232F) and a loxP site flanked neomycin resistance gene cassette was inserted into intron 28. The neo cassette was removed through subsequent Cre-mediated recombination. The mutations block phosphorylation of the affected residues in the encoded peptide. |
