| First Author | Degen J | Year | 2012 |
| Journal | FASEB J | Volume | 26 |
| Issue | 11 | Pages | 4576-83 |
| PubMed ID | 22859373 | Mgi Jnum | J:193352 |
| Mgi Id | MGI:5468218 | Doi | 10.1096/fj.12-207183 |
| Citation | Degen J, et al. (2012) Dual reporter approaches for identification of Cre efficacy and astrocyte heterogeneity. FASEB J 26(11):4576-83 |
| abstractText | Gene inactivation reporters are powerful tools to circumvent limitations of the widely used Cre/loxP system of conditional mutagenesis. With new conditional transgenic mouse lines expressing the enhanced cyan fluorescent protein (ECFP) instead of connexin43 (Cx43) after Cre-mediated recombination, we demonstrate dual reporter approaches to simultaneously examine astrocyte subpopulations expressing different connexins, identify compensatory up-regulation within gene families, and quantify Cre-mediated deletion at the allelic level. Analysis of a newly generated Cx43 knock-in ECFP mouse revealed an unexpected heterogeneity of Cx43-expressing astrocytes across brain areas. |
