| First Author | Seneschal J | Year | 2014 |
| Journal | J Invest Dermatol | Volume | 134 |
| Issue | 3 | Pages | 686-694 |
| PubMed ID | 24126845 | Mgi Jnum | J:206203 |
| Mgi Id | MGI:5548066 | Doi | 10.1038/jid.2013.418 |
| Citation | Seneschal J, et al. (2014) Langerin(+) Dermal DC, but Not Langerhans Cells, Are Required for Effective CD8-Mediated Immune Responses after Skin Scarification with Vaccinia Virus. J Invest Dermatol 134(3):686-94 |
| abstractText | Skin scarification (s.s.) with vaccinia virus (VACV) is essential for generation of an optimal protective T-cell memory immune response. Dendritic cells (DCs), which are professional antigen-presenting cells, are required for naive T-cell priming and activation. At least three subsets of skin-resident DC have been identified: Langerhans cells (LCs), dermal Langerin(+) DC (Lang(+)dDC), and dermal Langerin(-) DC (Lang(-)dDC). Using Langerin-diphtheria toxin receptor mice and established mouse model of VACV delivered by s.s., we demonstrated that Lang(+)dDC, but not LC, are absolutely required for the induction of a rapid and robust antigen-specific CD8(+) T-cell response after s.s. with VACV. The depletion of Lang(+)dDC led to a significant delay in the priming and proliferation of antigen-specific CD8(+) T cells. Moreover, CD8(+) T cells generated after VACV s.s. in the absence of Lang(+)dDC lacked effector cytotoxic functions both in vitro and in vivo. While s.s.-immunized wild-type and LC-depleted mice controlled the progression of OVA257-264 expressing T-cell lymphoma EG7 (injected intradermally), the depletion of Lang(+)dDC led to rapid lymphoma progression and mortality. These data indicate that of all skin DC subsets, Lang(+)dDC is the most critical for the generation of robust CD8(+) T-cell immunity after s.s. with VACV. |
