Primary Identifier | MGI:6119581 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Cluh |
Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted into intron 9 and an FRT site flanked neomycin resistance gene cassette and a second loxP site into intron 10. The neo cassette was removed through subsequent flp-mediated recombination and exon 10 was deleted through subsequent cre-mediated recombination. Western blots confirmed the absence of protein expression from this null allele. |