Primary Identifier | MGI:5552747 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Snord50a |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The first snoRNA 50 (mU50) sequence was replaced with a 68 bp multiple cloning site from pBluescriptII KSII plasmid containing an EcoRV site. The second mU50 site was replaced with a floxed neomycin resistance cassette. Cre-mediated recombination removed the resistance cassette. Northern blot analysis confirmed the absence of expression in the spleen. |