| First Author | Wang VE | Year | 2004 |
| Journal | Proc Natl Acad Sci U S A | Volume | 101 |
| Issue | 7 | Pages | 2005-10 |
| PubMed ID | 14762167 | Mgi Jnum | J:88356 |
| Mgi Id | MGI:3032859 | Doi | 10.1073/pnas.0307304101 |
| Citation | Wang VE, et al. (2004) B cell development and immunoglobulin transcription in Oct-1-deficient mice. Proc Natl Acad Sci U S A 101(7):2005-10 |
| abstractText | The POU domain transcription factors Oct-1 and Oct-2 interact with the octamer element, a motif conserved within Ig promoters and enhancers, and mediate transcription from the Ig loci. Inactivation of Oct-2 by gene targeting results in normal B cell development and Ig transcription. To study the role of Oct-1 in these processes, the lymphoid compartment of RAG-1(-/-) animals was reconstituted with Oct-1-deficient fetal liver hematopoietic cells. Recipient mice develop B cells with levels of surface Ig expression comparable with wild type, although at slightly reduced numbers. These B cells transcribe Ig normally, respond to antigenic stimulation, undergo class switching, and use a normal repertoire of light chain variable segments. However, recipient mice show slight reductions in serum IgM and IgA. Thus, the Oct-1 protein is dispensable for B cell development and Ig transcription. |
