| Primary Identifier | MGI:5789789 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mir127 |
| Transmission | Germline | Strain of Origin | 129 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The microRNA sequence was replaced with a floxed neomycin resistance cassette. Cre-mediated recombination removed the selection cassette. The deletion results in a nonsense mutation in exon 3 and a premature stop codon on the opposite strand in Rtl1. Western blot analysis confirmed the absence of Rtl1 protein expression in conceptuses. |
