Primary Identifier | MGI:3706252 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Trim54 |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exon 4 was replaced with a neomycin cassette, resulting in the splicing of exon 3 to exon 5. The sequences encoding the first coiled-coil domain were removed from the locus. RT-PCR demonstrated that the mutation eliminated nearly all detectable mRNA downstream of the neo cassette. |