First Author | Pettitt SJ | Year | 2009 |
Journal | Nat Methods | Volume | 6 |
Issue | 7 | Pages | 493-5 |
PubMed ID | 19525957 | Mgi Jnum | J:149352 |
Mgi Id | MGI:3848338 | Doi | 10.1038/nmeth.1342 |
Citation | Pettitt SJ, et al. (2009) Agouti C57BL/6N embryonic stem cells for mouse genetic resources. Nat Methods :1-3 |
abstractText | We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background. |