| First Author | Williamson CM | Year | 1994 |
| Journal | Genomics | Volume | 22 |
| Issue | 1 | Pages | 240-2 |
| PubMed ID | 7959780 | Mgi Jnum | J:19417 |
| Mgi Id | MGI:67587 | Doi | 10.1006/geno.1994.1373 |
| Citation | Williamson CM, et al. (1994) Protective protein for beta-galactosidase, Ppgb, maps to the distal imprinting region of mouse chromosome 2 but is not imprinted. Genomics 22(1):240-2 |
| abstractText | Human chromosome 20 is conserved as a single segment on distal mouse chromosome (Chr) 2. PPGB, protective protein for beta-galactosidase, maps to human chromosome 20q13.1, and from linkage analysis of two interspecific crosses incorporating the mouse reciprocal translocations, T(2;8)2Wa (T2Wa) and T(2;16)28H (T28H), we have mapped the mouse homologue, Ppgb, to the conserved region on distal mouse Chr 2. From the combined data, the order of markers is T2Wa-Ada-Ppgb-D2Mit25-T28H. Loci mapping between the T2Wa and T28H breakpoints lie in a region that is subject to parental imprinting, and so expression of Ppgb was tested in mice with maternal duplication/paternal deficiency and its reciprocal for the distal region of Chr 2. We have shown by reverse transcription-polymerase chain reaction (RT-PCR) that both parental alleles of Ppgb were expressed in the brain and kidney of 17.5-day-old embryos, 18.5-day-old embryos, and newborn mice. Thus, Ppgb does not appear to be subject to imprinting effects in the mouse. |
