| First Author | Maggio-Price L | Year | 1993 |
| Journal | Cell Immunol | Volume | 148 |
| Issue | 2 | Pages | 346-56 |
| PubMed ID | 7684329 | Mgi Jnum | J:4748 |
| Mgi Id | MGI:53231 | Doi | 10.1006/cimm.1993.1117 |
| Citation | Maggio-Price L, et al. (1993) Increased proliferative capacity of CD4+ and CD8+ T lymphocytes from mutant sphha/sphha mice is associated with increased IL-2 receptor expression. Cell Immunol 148(2):346-56 |
| abstractText | We previously discovered that mutant anemic mice (sphha/sphha) show increased numbers of cycling lymph node T lymphocytes when analyzed by pulse and continuous infusion of tritiated thymidine. We have now further analyzed this in vivo phenomenon by evaluating the in vitro proliferative response of anti-CD3 activated lymphocytes from anemic mice using flow cytometric cell cycle analysis with 5'-bromodeoxyuridine and Hoechst dye. We determined that sorted CD4+ and CD8+ T lymphocytes from anemic mice have significantly greater proliferative capacity when compared with syngeneic control (+/+) mice (P < 0.001). In order to explain this increased growth capacity, we examined whether these cells exhibit differences in cell-surface phenotype (Pgp-1 and IL-2 receptor expression), activation state, or transmembrane signaling, or alterations in accessory cells or cytokines. Increased proliferation of T cells from anemic mice was associated with a larger percentage of T cells expressing IL-2R (p55 or CD25) at 24 and 48 hr after activation. Increased proliferative capacity was not associated with differences in activation state, Pgp-1 phenotype, transmembrane signaling, accessory cells, or cytokines. The mechanism for the abnormally high proliferative rate of T cells from anemic mice remains unclear, but we suggest that this mutant mouse may provide an important model for further studies on the molecular basis of T-cell replication. |
