| First Author | Cattanach BM | Year | 1986 |
| Journal | Mouse News Lett | Volume | 74 |
| Pages | 94-95 | Mgi Jnum | J:14078 |
| Mgi Id | MGI:62255 | Citation | Cattanach BM, et al. (1986) X chromosomal location of Sts. Mouse News Lett 74:94-95 |
| abstractText | Full text of MNL contribution: Linkage data. 2. X chromosomal location of Sts. Evidence for the location of Sts has been ambiguous; two studies using a low activity variant of A/J mice have produced segregation data compatible with an autosomal inheritance, but two others using a deficiency variant have suggested X- or X, Y-linkage (Kietges et al., Nature 315:226-227, 1985). In man,an Sts locus lies distally in the short arm of the X close to the X-Y pairing region. In the mouse, therefore, such a locus might be located at the distal end of the acrocentric X near the region of X-Y crossing over. Linkage between the low activity A/J variant and the distal X chromosome marker Crm has now been sought in backcrosses of Crm Sts-high/+ Stslow, cc females x + Sts-high/Y, cc males. A bimodal distribution of liver Sts activities was found among the male progeny. Significantly, all but 2 of 23 phenotypically Crm males assayed had high Sts activities within the range found for mice of the ancestral high Sts stock (overall mean 15.50.8 c +/- 0.8 c.f. 14.5 +/- 1.2 nanomoles/mg protein/hour), whereas all but 3 of 23 phenotypically + males assayed had low activities within the range found for A/J mice (overall mean 11.4 +/- 0.7 cf 12.4 +/- 0.9). It is not known whether the 5 exceptional progeny represented recombinants or only extremes of the two activity distributions. However, the data clearly demonstrate that the Sts locus is X-linked in accord with Ohno's postulate of the evolutionary conservation of the mammalian X, and support Keitges et al's (1985) prediction that Sts should be located at the distal, X-Y pairing end of the chromosome. Comparison of the + backcross and A/J male Sts activities has not provided any indication that an homologous locus on the Y is expressed. We thank Dr. E. Simpson for providing the A/J mice. (Cattanach and Crocker, Churchill Hospital, Oxford) |
