| First Author | Leiter EH | Year | 1979 |
| Journal | In Vitro | Volume | 15 |
| Issue | 7 | Pages | 507-21 |
| PubMed ID | 393618 | Mgi Jnum | J:6264 |
| Mgi Id | MGI:54741 | Doi | 10.1007/BF02618153 |
| Citation | Leiter EH, et al. (1979) Endocrine pancreatic cells of postnatal diabetes (db) mice in cell culture. In Vitro 15(7):507-21 |
| abstractText | Ultrastructural characteristics as well as secretory and biosynthetic behavior of monolayer pancreatic cell cultures established from 4-day-old C57BL/KsJ misty diabetic (m db/m db) mice have been studied in comparison to normal littermate controls. Hypersecretion of glucagon by alpha-cells from BL/Ks misty diabetic mice after 2 days in vitro was found to precede any hyperfunction of the insulin-secreting beta-cells. The increased level of glucagon-release in BL/Ks cell cultures from diabetic mice was accompanied by a greatly enhanced level of incorporation of [3H]tryptophan into glucagon-like molecules whose specific radioactivity was up to 15-fold higher than that observed in cultures from genetic controls. The finding of an alpha-cell dysfunction in cultures established from preweaning diabetic BL/Ks mice suggests that glucagon could play an early role in shaping the events that culminate in the expression of frank diabetes in this inbred strain. |
