| First Author | Wu AL | Year | 2011 |
| Journal | Sci Transl Med | Volume | 3 |
| Issue | 113 | Pages | 113ra126 |
| PubMed ID | 22174314 | Mgi Jnum | J:183639 |
| Mgi Id | MGI:5319030 | Doi | 10.1126/scitranslmed.3002669 |
| Citation | Wu AL, et al. (2011) Amelioration of type 2 diabetes by antibody-mediated activation of fibroblast growth factor receptor 1. Sci Transl Med 3(113):113ra126 |
| abstractText | Clinical use of recombinant fibroblast growth factor 21 (FGF21) for the treatment of type 2 diabetes and other disorders linked to obesity has been proposed; however, its clinical development has been challenging owing to its poor pharmacokinetics. Here, we describe an alternative antidiabetic strategy using agonistic anti-FGFR1 (FGF receptor 1) antibodies (R1MAbs) that mimic the metabolic effects of FGF21. A single injection of R1MAb into obese diabetic mice induced acute and sustained amelioration of hyperglycemia, along with marked improvement in hyperinsulinemia, hyperlipidemia, and hepatosteatosis. R1MAb activated the mitogen-activated protein kinase pathway in adipose tissues, but not in liver, and neither FGF21 nor R1MAb improved glucose clearance in lipoatrophic mice, which suggests that adipose tissues played a central role in the observed metabolic effects. In brown adipose tissues, both FGF21 and R1MAb induced phosphorylation of CREB (cyclic adenosine 5'-monophosphate response element-binding protein), and mRNA expression of PGC-1alpha (peroxisome proliferator-activated receptor-gamma coactivator 1alpha) and the downstream genes associated with oxidative metabolism. Collectively, we propose FGFR1 in adipose tissues as a major functional receptor for FGF21, as an upstream regulator of PGC-1alpha, and as a compelling target for antibody-based therapy for type 2 diabetes and other obesity-associated disorders. |
