| First Author | Sakai T | Year | 2009 |
| Journal | Biochem Biophys Res Commun | Volume | 384 |
| Issue | 2 | Pages | 173-9 |
| PubMed ID | 19401193 | Mgi Jnum | J:152435 |
| Mgi Id | MGI:4358682 | Doi | 10.1016/j.bbrc.2009.04.105 |
| Citation | Sakai T, et al. (2009) Up-regulation of protease-activated receptor-1 in diabetic glomerulosclerosis. Biochem Biophys Res Commun 384(2):173-9 |
| abstractText | Patients with diabetes are under a hypercoagulable state leading to generation of thrombin. It is not known whether thrombin plays a role in the progression of diabetic nephropathy. We analyzed gene expression of two thrombin receptors, protease-activated receptor-1 (PAR-1) and PAR-4 in the kidney of diabetic db/db mice. Mice developed hyperglycemia from 7 to 10 weeks of age and showed renal abnormalities such as mesangial expansion and urinary albumin excretion at 10 weeks of age. PAR-1 mRNA was up-regulated in isolated glomeruli in db/db mice compared with age-matched db/m littermates, but PAR-4 mRNA was not. In situ hybridization studies showed that PAR-1 mRNA was detected mainly at the glomerulus, and that intensive signals were observed in mesangial cells and podocytes. The up-regulation of PAR-1 in glomeruli in diabetic mice may play a role in the progression of glomerulosclerosis and abnormal urinary albumin excretion in diabetic nephropathy. |
