| First Author | Neumann P | Year | 1988 |
| Journal | Mouse News Lett | Volume | 81 |
| Pages | 59 | Mgi Jnum | J:14140 |
| Mgi Id | MGI:62315 | Citation | Neumann P, et al. (1988) An insertional mutation causing neurologic dysfunction and reduced male fertility in a transgenic mouse. Mouse News Lett 81:59 |
| abstractText | Full text of MNL contribution: Research News: 1. An insertional mutation causing neurologic dysfunction and reduced male fertility in a transgenic mouse. We have identified a transgenic mouse pedigree (p447) in which homozygotes for the foreign gene manifest a neurologic disorder and reduced male fertility in vivo and in vitro. The mutation was identified in a mouse pedigree established by microinjection of pFR400, a 4.4 kilobase recombinant plasmid carrying an altered dihydrofolate reductase gene. Mutants appear normal until 12-15 days after birth, when they develop a markedly ataxic gait. By 25 days of age, mutants have rapid alternating patting motions of all four limbs. During locomotion, there are alternating stereotypic jerky flexions of the limbs, most pronounced in the hind limbs. The insertional mutation in p447 mice is allelic to hotfoot (ho), which maps to chromosome 6 (MNL 64:60, 1981). Neuropathologic changes have not been found in p447 or ho4J homozygotes. Several alleles of ho produce sterility in male homozygotes (MNL 34:30, 1966), however males homozygous for the only extant allele (ho4J) are fertile. (Paul Neumann with Jon W. Gordon, Joan Uehlinger and Beth E. Talansky, Mt. Sinai School of Medicine, New York). |
