| First Author | Hoshino Y | Year | 2017 |
| Journal | Exp Anim | Volume | 66 |
| Issue | 4 | Pages | 437-445 |
| PubMed ID | 28717054 | Mgi Jnum | J:259614 |
| Mgi Id | MGI:6149118 | Doi | 10.1538/expanim.17-0049 |
| Citation | Hoshino Y, et al. (2017) Simple generation of hairless mice for in vivo imaging. Exp Anim 66(4):437-445 |
| abstractText | The in vivo imaging of mice makes it possible to analyze disease progress non-invasively through reporter gene expression. As the removal of hair improves the accuracy of in vivo imaging, gene-modified mice with a reporter gene are often crossed with Hos:HR-1 mutant mice homozygous for the spontaneous Hr(hr) mutation that exhibit a hair loss phenotype. However, it is time consuming to produce mice carrying both the reporter gene and mutant Hr(hr) gene by mating. In addition, there is a risk that genetic background of the gene-modified mice would be altered by mating. To resolve these issues, we established a simple method to generate hairless mice maintaining the original genetic background by CRISPR technology. First, we constructed the pX330 vector, which targets exon 3 of Hr. This DNA vector (5 ng/microl) was microinjected into the pronuclei of C57BL/6J mice. Induced Hr gene mutations were found in many founders (76.1%) and these mutations were heritable. Next, we performed in vivo imaging using these gene-modified hairless mice. As expected, luminescent objects in their body were detected by in vivo imaging. This study clearly showed that hairless mice could be simply generated by the CRISPR/Cas9 system, and this method may be useful for in vivo imaging studies with various gene-modified mice. |
