| First Author | Meyers RS | Year | 1976 |
| Journal | Genetics | Volume | 83 |
| Pages | S50 (Abstr.) | Mgi Jnum | J:30708 |
| Mgi Id | MGI:78423 | Citation | Meyers RS, et al. (1976) Altered DNA content and chromatin distribution associated with the mouse ichthyosis gene. Genetics 83:S50 (Abstr.) |
| abstractText | Full text of Abstract: 50. ABSTRACTS. MEYERS, ROSALIE S., ABRAHAM S. KLEIN, JOHN J. EPPIG and RONALD A. ECKHARDT, Brooklyn College of The City University of New York, Brooklyn, NY and The Jackson Laboratory, Bar Harbor, ME. Altered DNA content and chromatin distribution associated with the mouse ichthyosis gene. - In addition to its phenotypic effects at the organismic level, the genetic mutation ichthyosis (ic) in mice is associated with an altered pattern of chromatin distribution in the nuclei of a variety of tissues. This altered pattern appears to exist in all tissues examined, but is particularly prominent in the cells of the thymus. Interphase chromatin of cells from normal mice usually is evenly distributed throughout the nucleus with the heterochromatic portion tightly associated with the nuclear membrane. In contrast to the normal interphase pattern, ic/ic cells display several large foci of chromatin condensation in the central nuclear region. Using the electron microscope, we have found in these cells a lessened degree of hetrochromatin attachment to the nuclear membrane. Microspectrophotometric measurements of +/+ and ic/ic thymocytes from adult males indicate that the ic/ic cells have about 10% more DNA than do +/+ cells. Measurements of spermatid nuclei give similar results. The DNA content of +/ic spermatids is intermediate to that of +/+ and ic/ic cells. Comparisons of the histone content between normal and mutant cells from a variety of tissues show minor quantitative differences, but no detectable qualitative differences. We are presently exploring the possibility that there may be differences in the repetitive DNA content of the two cell types. (Supported by NIH grant HD-05944 and FRAP 11066 from the Research Foundation-CUNY.) |
