| First Author | Vouyiouklis DA | Year | 2000 |
| Journal | J Neurochem | Volume | 74 |
| Issue | 3 | Pages | 940-8 |
| PubMed ID | 10693924 | Mgi Jnum | J:60320 |
| Mgi Id | MGI:1353166 | Doi | 10.1046/j.1471-4159.2000.0740940.x |
| Citation | Vouyiouklis DA, et al. (2000) A proteolipid protein-specific pre-mRNA (Ppm-1) contains intron 3 and is up-regulated during myelination in the CNS. J Neurochem 74(3):940-8 |
| abstractText | Alternative splicing of the precursor for messenger RNA (pre-mRNA) is a common process utilised by higher eukaryotes to modulate gene expression. A single primary transcript may generate several proteins with distinct functions, expressed in tissue-specific, developmental patterns. This article describes an oligodendrocyte-specific pre-mRNA product of proteolipid protein gene (P/p) transcription, which is the precursor for P/p but not Dm20 mRNA in the CNS. This P/p-specific pre-mRNA (Ppm-1) includes the intact intron 3 of the P/p gene. It is first expressed during active myelination, and it localises to the nucleus of oligodendrocytes, in both normal and jimpy (jp) murine CNS. In addition to mouse, Ppm-1 is found also in rat and dog, but not toad or trout. Our work suggests that alternative splicing of the P/p gene primary transcript follows a branching pattern, resulting in the presence of at least one P/p isoform-specific pre-mRNA molecule, Ppm-1. Therefore, Dm20 mRNA may be the product of a divergent set of pre-mRNA splicing events. |
