| First Author | Buehr M | Year | 1993 |
| Journal | Dev Dyn | Volume | 198 |
| Issue | 3 | Pages | 182-9 |
| PubMed ID | 8136523 | Mgi Jnum | J:16216 |
| Mgi Id | MGI:64302 | Doi | 10.1002/aja.1001980304 |
| Citation | Buehr M, et al. (1993) Proliferation and migration of primordial germ cells in We/We mouse embryos. Dev Dyn 198(3):182-9 |
| abstractText | We have examined the numbers and distribution of primordial germ cells in We/We, We/+, and +/+ mouse embryos using Southern blotting to determine embryo genotypes. At early somite stages (5-7 somites: approximately 8 1/2 days post coitum [dpc]) there are 50 to 100 germ cells in embryos of all genotypes. The number of germ cells in We/+ and +/+ embryos then begins to increase: at later somite stages (17-19 somites: approximately 9 1/2 dpc) they number about 200, and by 10 1/2 dpc there are approximately 725 We/+ and 850 +/+ germ cells. During this time, however, the number of germ cells in We/We embryos remains less than 100. At 8 1/2 dpc, the distribution of germ cells in the hindgut endoderm is the same in all genotypes. By 9 1/2 dpc, 30% of We/We germ cells are found in ectopic sites (allantois and vitelline artery); germ cell distribution along the length of the hindgut appears normal, but germ cells remain confined to the floor of the gut in We/We embryos, rather than being distributed around its circumference as in the other two genotypes. By 10 1/2 days, the migration of We/We germ cells through the dorsal mesentery lags behind that of the other genotypes, and a larger proportion remains in the gut wall. |
