| First Author | Nagai R | Year | 2012 |
| Journal | Dev Dyn | Volume | 241 |
| Issue | 8 | Pages | 1374-84 |
| PubMed ID | 22745058 | Mgi Jnum | J:185654 |
| Mgi Id | MGI:5429626 | Doi | 10.1002/dvdy.23824 |
| Citation | Nagai R, et al. (2012) Dynamics of GFRalpha1-positive spermatogonia at the early stages of colonization in the recipient testes of W/W(nu) male mice. Dev Dyn 241(8):1374-84 |
| abstractText | Background: The spermatogonial transplantation experiment can be used as an unequivocal detection assay of spermatogenic stem cells (SSCs) in both a qualitative and quantitative manner, based on their regenerative capacity. In this study, the proliferative patterns and kinetics of donor-derived GFRalpha1-positive spermatogonia containing potential SSCs were examined during early colonization following spermatogonial transplantation.Results: Donor-derived GFRalpha1-positive cells frequently formed several aggregates of A(al(aligned)) /morula-like structures in a single spermatogenic cell patch before and on day 14 post-transplant, indicating a possible involvement in the formation of a stable spermatogenic colony at 21 days post-transplant. The appearance of these A(al) /morula-like aggregates is positively correlated with regional, high-level expression of immunoreactive GDNF signals, a ligand for GFRalpha1, associated with colony expansion. Conclusions: These data raise the hypothesis that regional GDNF signals regulate the balance between donor-derived A(al) -like cell aggregates and their differentiation in each small patch, which subsequently leads to further selection of survival colonies at later stages. Developmental Dynamics 241:1374-1384, 2012. (c) 2012 Wiley Periodicals, Inc. |
