| First Author | Choi IH | Year | 1998 |
| Journal | J Exp Med | Volume | 188 |
| Issue | 9 | Pages | 1587-92 |
| PubMed ID | 9802970 | Mgi Jnum | J:50773 |
| Mgi Id | MGI:1309711 | Doi | 10.1084/jem.188.9.1587 |
| Citation | Choi IH, et al. (1998) Immunoglobulin E-dependent active fatal anaphylaxis in mast cell-deficient mice. J Exp Med 188(9):1587-92 |
| abstractText | Mast cells have long been believed to be the central effector cells in the development of immunoglobulin (Ig)E-dependent anaphylaxis. In this study, we investigated the role of mast cells in IgE-dependent hapten-induced active fatal anaphylaxis using mast cell-deficient WBB6F1- W/Wv (W/Wv) and congenic normal (+/+) mice. Although a 5-min delay in shock signs and death were observed in W/Wv mice, 100% fatal reactions to penicillin V (Pen V) occurred in both +/+ and W/Wv mice. Administration of monoclonal anti-IL-4 antibody completely prevented the fatal reactions, and the effect of anti-IL-4 was associated with its suppressive activity on Pen V-specific serum levels of IgE, but not IgG. The platelet-activating factor (PAF) antagonist, BN 50739, completely prevented the fatal reactions in both strains of mice. Our kinetic study revealed, in contrast to no elevation of plasma histamine level in W/Wv mice, high levels of PAF in the circulation after challenge in both +/+ and W/Wv mice, albeit to a lesser degree in the latter case. These data indicate that cells other than mast cells are sufficient to induce an IgE-dependent active fatal anaphylaxis by elaborating PAF, which is the critical mediator for fatal murine anaphylaxis. |
