| First Author | Dastych J | Year | 1998 |
| Journal | Am J Physiol | Volume | 275 |
| Issue | 5 Pt 1 | Pages | C1291-9 |
| PubMed ID | 9814978 | Mgi Jnum | J:50928 |
| Mgi Id | MGI:1313053 | Doi | 10.1152/ajpcell.1998.275.5.C1291 |
| Citation | Dastych J, et al. (1998) Tyrosine kinase-deficient Wv c-kit induces mast cell adhesion and chemotaxis. Am J Physiol 275(5 Pt 1):C1291-9 |
| abstractText | W/Wv mice are deficient in tissue mast cells, and mast cells cultured from these mice do not proliferate in response to the c-kit ligand, stem cell factor (SCF). In this paper, we report that mouse bone marrow cultured mast cells derived from W/Wv mice do adhere to fibronectin in the presence of SCF and exhibit chemotaxis to SCF, and we explore this model for the understanding of c-kit-mediated signaling pathways. Both in vitro and in vivo (in intact cells) phosphorylation experiments demonstrated a low residual level of W/Wv c-kit protein phosphorylation. SCF-induced responses in W/Wv mast cells were abolished by the tyrosine kinase inhibitor herbimycin A and by the phospa-tidylinositol 3-kinase (PI 3-kinase) inhibitor wortmannin but were not affected by protein kinase C inhibitors. These observations are consistent with the conclusions that Wv c-kit initiates a signaling process that is PI 3-kinase dependent and that mutated Wv c-kit retains the ability to initiate mast cell adhesion and migration. |
