| First Author | Cole JD | Year | 2022 |
| Journal | Exp Eye Res | Volume | 218 |
| Pages | 109012 | PubMed ID | 35245513 |
| Mgi Jnum | J:322249 | Mgi Id | MGI:7257626 |
| Doi | 10.1016/j.exer.2022.109012 | Citation | Cole JD, et al. (2022) Long-term retinal protection by MEK inhibition in Pax6 haploinsufficiency mice. Exp Eye Res 218:109012 |
| abstractText | Aniridia is a panocular condition characterized by impaired eye development and vision, which is mainly due to the haploinsufficiency of the paired-box-6 (PAX6) gene. Like what is seen in aniridia patients, Pax6-deficient mice Pax6(Sey)(-Neu/+) exhibit a varied degree of ocular damage and impaired vision. Our previous studies showed that these phenotypes were partially rescued by PD0325901, a mitogen-activated protein kinase kinase (MEK or MAP2K) inhibitor. In this study, we assessed the long-term efficacy of PD0325901 treatment in retinal health and visual behavior. At about one year after the postnatal treatment with PD0325901, Pax6(Sey-Neu/+) mice showed robust improvements in retina size and visual acuity, and the elevated intraocular pressure (IOP) was also alleviated, compared to age-matched mice treated with vehicles only. Moreover, the Pax6(Sey-Neu/+) eyes showed disorganized retinal ganglion cell (RGC) axon bundles and retinal layers, which we termed as hotspots. We found that the PD treatment reduced the number and size of hotspots in the Pax6(Sey-Neu/+) retinas. Taken together, our results suggest that PD0325901 may serve as an efficacious intervention in protecting retina and visual function in aniridia-afflicted subjects. |
