| First Author | Rowan S | Year | 2010 |
| Journal | Genes Dev | Volume | 24 |
| Issue | 10 | Pages | 980-5 |
| PubMed ID | 20413611 | Mgi Jnum | J:160146 |
| Mgi Id | MGI:4453504 | Doi | 10.1101/gad.1890410 |
| Citation | Rowan S, et al. (2010) Precise temporal control of the eye regulatory gene Pax6 via enhancer-binding site affinity. Genes Dev 24(10):980-5 |
| abstractText | How transcription factors interpret the cis-regulatory logic encoded within enhancers to mediate quantitative changes in spatiotemporally restricted expression patterns during animal development is not well understood. Pax6 is a dosage-sensitive gene essential for eye development. Here, we identify the Prep1 (pKnox1) transcription factor as a critical dose-dependent upstream regulator of Pax6 expression during lens formation. We show that Prep1 activates the Pax6 lens enhancer by binding to two phylogenetically conserved lower-affinity DNA-binding sites. Finally, we describe a mechanism whereby Pax6 levels are determined by transcriptional synergy of Prep1 bound to the two sites, while timing of enhancer activation is determined by binding site affinity. |
