| First Author | Yang-Feng TL | Year | 1987 |
| Journal | Cytogenet Cell Genet | Volume | 46 |
| Pages | 723 (Abstr.) | Mgi Jnum | J:12308 |
| Mgi Id | MGI:60556 | Citation | Yang-Feng TL, et al. (1987) The oncogene c-kit (KIT) is located on human Chromosome 4 and mouse Chromosome 5. Cytogenet Cell Genet 46:723 (Abstr.) |
| abstractText | Full text of Abstract: Abstracts of workshop presentations. The oncogene c-kit (KIT) is located on human chromosome 4 and mouse chromosome 5. Yang-Feng TL,1 Ullrich A,2 Francke U.1 1Department of Human Genetics, Yale University School of Medicine, New Haven, CT. 2Department of Developmental Biology, Genentech, Inc., South San Francisco, CA. The c-kit oncogene encodes a 145 kDa transmembrane glycoprotein that is capable of self- phosphorylation on tyrosine residues in both human glioblastoma cells and transfected mouse fibroblasts. It is structurally related to the receptors for macrophage colony stimulating factor (c-fms) and for platelet-derived growth factor (PDGFR) and probably functions as a cell surface receptor. A 900 bp human c-kit cDNA clone was used to localize the c-kit locus in human and mouse. Analysis of 233 grains in 100 metaphases after in situ chromosomal hybridization revealed that 9.5% of total silver grains (22/233) were over bands q11-q21 of human chromosome 4. However, 7.9% grains were found at a second site on chromosome 5, 5q23-q34. This second site of hybridization coincides with the location of related receptor genes, including PDGFR and c-fms/CSF-1 receptor. Southern analysis of DNA from 25 somatic cell hybrids confirmed the assignment of c-kit to human chromosome 4. Two PvuII fragments or two EcoRI fragments were detected in human control DNA and in DNA of hybrids containing human chromosome 4. Since both PvuII or both EcoRI fragments were present in 11 positive hybrids, there was no evidence for a second cross-hybridizing sequence on filter hybridization which is done at a considerably higher stringency than in situ hybridization. All other chromosomes have been ruled out as carrying c-kit sequences by at least 5 discordant hybrids. A panel of 11 Chinese hamster x mouse hybrids and one rat x mouse hybrid was used to map c-kit to mouse chromosome 5. Every other mouse chromosome was excluded by two or more discordant hybrids. The assignment of the c-kit locus to mouse chromosome 5 adds one more gene to the known syntenic groups on the proximal long arm of human chromosome 4 and on mouse chromosome 5. HGM symbol : KIT |
