| First Author | Drissen R | Year | 2016 |
| Journal | Nat Immunol | Volume | 17 |
| Issue | 6 | Pages | 666-676 |
| PubMed ID | 27043410 | Mgi Jnum | J:260178 |
| Mgi Id | MGI:6140681 | Doi | 10.1038/ni.3412 |
| Citation | Drissen R, et al. (2016) Distinct myeloid progenitor-differentiation pathways identified through single-cell RNA sequencing. Nat Immunol 17(6):666-676 |
| abstractText | According to current models of hematopoiesis, lymphoid-primed multi-potent progenitors (LMPPs) (Lin(-)Sca-1(+)c-Kit(+)CD34(+)Flt3(hi)) and common myeloid progenitors (CMPs) (Lin(-)Sca-1(+)c-Kit(+)CD34(+)CD41(hi)) establish an early branch point for separate lineage-commitment pathways from hematopoietic stem cells, with the notable exception that both pathways are proposed to generate all myeloid innate immune cell types through the same myeloid-restricted pre-granulocyte-macrophage progenitor (pre-GM) (Lin(-)Sca-1(-)c-Kit(+)CD41(-)FcgammaRII/III(-)CD150(-)CD105(-)). By single-cell transcriptome profiling of pre-GMs, we identified distinct myeloid differentiation pathways: a pathway expressing the gene encoding the transcription factor GATA-1 generated mast cells, eosinophils, megakaryocytes and erythroid cells, and a pathway lacking expression of that gene generated monocytes, neutrophils and lymphocytes. These results identify an early hematopoietic-lineage bifurcation that separates the myeloid lineages before their segregation from other hematopoietic-lineage potential. |
