| First Author | Hirasawa T | Year | 1997 |
| Journal | Exp Anim | Volume | 46 |
| Issue | 1 | Pages | 75-8 |
| PubMed ID | 9027475 | Mgi Jnum | J:41265 |
| Mgi Id | MGI:893344 | Doi | 10.1538/expanim.46.75 |
| Citation | Hirasawa T, et al. (1997) Genetic typing of the mouse ob mutation by PCR and restriction enzyme analysis. Exp Anim 46(1):75-8 |
| abstractText | A genetic typing method for the mouse obese (ob) mutation by PCR and restriction fragment length polymorphism (RFLP) analysis was developed. Three genotypes (ob/ob, ob/+ and +/+) of the ob mouse are rapidly differentiated with this assay. Since only a small biopsy specimen (tail end) is necessary for the genotyping, the ob mouse at any age is typable and can be used in subsequent breeding or research. Obese homozygotes (ob/ob) were efficiently produced by mating heterozygotes (ob/+) only, which were selected by using the PCR-RFLP assay. |
