| First Author | Oler AT | Year | 2008 |
| Journal | J Lipid Res | Volume | 49 |
| Issue | 5 | Pages | 1126-9 |
| PubMed ID | 18272929 | Mgi Jnum | J:133040 |
| Mgi Id | MGI:3777622 | Doi | 10.1194/jlr.D800002-JLR200 |
| Citation | Oler AT, et al. (2008) A rapid, microplate SNP genotype assay for the leptinob allele. J Lipid Res 49(5):1126-9 |
| abstractText | A nonsense mutation in the mouse leptin gene causes genetic obesity. As a result of extensive research in the field of obesity, the use of leptin(ob) mice is widespread. This mutation renders mice sterile, creating the need to breed heterozygous mice. For this reason, leptin(ob) genotyping is necessary. To date, gel-based assays have been used for genotyping. Using the Invader Plus(R) assay for single nucleotide polymorphism (SNP) detection, we have developed a gel-free microplate SNP assay for genotyping leptin(wt) and leptin(ob) alleles. |
