| First Author | Gustavsson N | Year | 2006 |
| Journal | Biochem Biophys Res Commun | Volume | 340 |
| Issue | 4 | Pages | 1119-24 |
| PubMed ID | 16414347 | Mgi Jnum | J:104995 |
| Mgi Id | MGI:3613264 | Doi | 10.1016/j.bbrc.2005.12.119 |
| Citation | Gustavsson N, et al. (2006) Timing of Ca2+ response in pancreatic beta-cells is related to mitochondrial mass. Biochem Biophys Res Commun 340(4):1119-24 |
| abstractText | The timing and magnitude of calcium response are cell-specific in individual beta-cells. This may indicate that the cells have different roles in the intact islet. It is unknown what mechanisms determine these characteristics. We previously found that the mechanisms setting cell-specific response timing are disturbed in beta-cells from hyperglycemic mice and one of the causes is likely to be an altered mitochondrial metabolism. Mitochondria play a key role in the control of nutrient-induced insulin secretion. Here, we used confocal microscopy with the fluorescent probe MitoTracker Red CMXRos and Fluo-3 to study how the amount of active mitochondria is related to the lag-time and the magnitude of calcium response to 20mM glucose in isolated beta-cells and in cells within intact lean and ob/ob mouse islets. Results show that the mitochondrial mass is inversely correlated with the lag-times for calcium response both in lean and ob/ob mouse beta-cells (r=-0.73 and r=-0.43, respectively, P<0.05). Thus, the state of mitochondria may determine the timing of calcium response. |
