| First Author | Harris RB | Year | 1998 |
| Journal | Endocrinology | Volume | 139 |
| Issue | 1 | Pages | 8-19 |
| PubMed ID | 9421392 | Mgi Jnum | J:44829 |
| Mgi Id | MGI:1101375 | Doi | 10.1210/endo.139.1.5675 |
| Citation | Harris RB, et al. (1998) A leptin dose-response study in obese (ob/ob) and lean (+/?) mice. Endocrinology 139(1):8-19 |
| abstractText | This experiment determined the amount of leptin required to correct different abnormalities in leptin-deficient ob/ob mice. Baseline food intakes and body weights of lean (+/?) and obese (ob/ob) C57B1/6J <ob> mice were recorded for 7 days. An Alzet miniosmotic pump was placed in the peritoneal cavity of each mouse and delivered 0, 1, 2, 5, 10, or 42 microg/day human leptin for 7 days. In ob/ob mice, 2 microg leptin/day reduced food intake and body weight, and increased hypothalamic and brain stem serotonin concentrations. All fat pads were reduced 35-40% by 10 microg leptin/day, and liver weight, lipid, and glycogen decreased. Serum insulin and glucose were reduced in all leptin-treated ob/ob mice, and levels were normalized by 10 microg/day leptin. Low rectal temperatures of ob/ob mice were corrected by 10 and 42 microg/day leptin. These doses also increased brown adipose tissue uncoupling protein expression. The only responses in lean mice were a transient reduction in food intake and weight loss with 10 or 42 microg/day leptin. This study shows enhanced leptin sensitivity in ob/ob mice and suggests that increased temperature and sympathetic activity are indirect responses to high concentrations of protein. |
