| First Author | Jones J | Year | 1988 |
| Journal | Mouse News Lett | Volume | 82 |
| Pages | 123 | Mgi Jnum | J:64098 |
| Mgi Id | MGI:1888720 | Citation | Jones J, et al. (1988) A viable deletion at albino. Tyr |
| abstractText | Full text of MNL contribution: A viable deletion at albino. J. Jones1, J. Peters1, S. Ball1, B.M. Cattanach1 and B.S. Kwon2. 1MRC Radiobiology Unit, Chilton, Didcot, Oxon, OX11 ORD; 2Guthrie Research Institute, Sayre, PA 18840, USA. The albino, c, locus on chromosome 7 of the mouse is thought to be the structural gene for tyrosinase. A number of deletions of the locus are known, all of which have proved to be homozygous lethal. We have used a mouse cDNA probe for tyrosinase, PMTY 118, to analyse DNA from three new c mutations, c44H, C45H, and C46H. All three mutants were induced in mouse spermatogonial stem cells by combined HU-X-ray treatment (Cattanach & Rasberry MNL 75:25, 1986). The mutants are fully fertile and viable when homozygous, but whereas c45H and c46H appear identical to c, c44H has dark eyes and traces of pigmented hair in the ano-genital area (Cattanach & Rasberry MNL 81: 64, 1988). Molecular analysis by Southern blotting of DNA from +c, c, cch and the three mutants has been carried out. Restriction fragment length polymorphism has been found between c, cch and +c mice, for with EcoRI an 11 kb band is found in c and cch replaced by a 14 kb band in +c. c44H and c46H showed the same restriction patterns as +c, and so these two mutants could have resulted from small intragenic changes. However, DNA from c45H did not hybridize to the tyrosinase probe, even though a number of restriction enzymes have been used. This indicates that the c45H mutation is a deletion which must be at least as large as the tyrosinase structural gene and its immediate flanking sequences. Thus, c45H is novel in that as a c deletion it is viable in the homozygous condition. |
