| First Author | Lindsley RC | Year | 2007 |
| Journal | Blood | Volume | 109 |
| Issue | 6 | Pages | 2521-8 |
| PubMed ID | 17105816 | Mgi Jnum | J:145687 |
| Mgi Id | MGI:3835758 | Doi | 10.1182/blood-2006-04-018085 |
| Citation | Lindsley RC, et al. (2007) Generation of peripheral B cells occurs via two spatially and temporally distinct pathways. Blood 109(6):2521-8 |
| abstractText | We have identified a population of newly formed bone marrow (BM) B cells that shares multiple characteristics with late transitional B cells in the spleen. Both late splenic transitional B cells and cells within this uncharacterized BM population expressed the cell-surface phenotype AA4(+) CD23(+), yet the developmental kinetics and the renewal rate of AA4(+) CD23(+) BM B cells mirrored recently formed BM B cells. Further, unlike the least mature B cells in the BM and spleen, AA4(+) CD23(+) BM B cells expressed the homing receptor CD62L, were dependent on the antiapoptotic cytokine receptor BR3 and the tec family kinase Btk, and proliferated in response to IL-4 plus CD40 stimulation. Finally, frequencies of lambda light chain-positive B cells declined among AA4(+) CD23(+) B cells in both the BM and spleen, suggesting that V-gene selection events correlate with CD23 expression in both compartments. These observations indicate that the first step in B-cell maturation occurs in both the BM and the periphery and suggest that recently formed B cells exit the BM as a heterogeneous pool of immature and semimature B cells. |
