| First Author | Fiore PF | Year | 2020 |
| Journal | Int J Mol Sci | Volume | 21 |
| Issue | 3 | PubMed ID | 32023816 |
| Mgi Jnum | J:298510 | Mgi Id | MGI:6480198 |
| Doi | 10.3390/ijms21030932 | Citation | Fiore PF, et al. (2020) Lack of PKCtheta Promotes Regenerative Ability of Muscle Stem Cells in Chronic Muscle Injury. Int J Mol Sci 21(3):932 |
| abstractText | Duchenne muscular dystrophy (DMD) is a genetic disease characterized by muscle wasting and chronic inflammation, leading to impaired satellite cells (SCs) function and exhaustion of their regenerative capacity. We previously showed that lack of PKCtheta in mdx mice, a mouse model of DMD, reduces muscle wasting and inflammation, and improves muscle regeneration and performance at early stages of the disease. In this study, we show that muscle regeneration is boosted, and fibrosis reduced in mdxtheta(-/-) mice, even at advanced stages of the disease. This phenotype was associated with a higher number of Pax7 positive cells in mdxtheta(-/-) muscle compared with mdx muscle, during the progression of the disease. Moreover, the expression level of Pax7 and Notch1, the pivotal regulators of SCs self-renewal, were upregulated in SCs isolated from mdxtheta(-/-) muscle compared with mdx derived SCs. Likewise, the expression of the Notch ligands Delta1 and Jagged1 was higher in mdxtheta(-/-) muscle compared with mdx. The expression level of Delta1 and Jagged1 was also higher in PKCtheta(-/-) muscle compared with WT muscle following acute injury. In addition, lack of PKCtheta prolonged the survival and sustained the differentiation of transplanted myogenic progenitors. Overall, our results suggest that lack of PKCtheta promotes muscle repair in dystrophic mice, supporting stem cells survival and maintenance through increased Delta-Notch signaling. |
