| First Author | Kuang S | Year | 2007 |
| Journal | Cell | Volume | 129 |
| Issue | 5 | Pages | 999-1010 |
| PubMed ID | 17540178 | Mgi Jnum | J:342148 |
| Mgi Id | MGI:7545378 | Doi | 10.1016/j.cell.2007.03.044 |
| Citation | Kuang S, et al. (2007) Asymmetric self-renewal and commitment of satellite stem cells in muscle. Cell 129(5):999-1010 |
| abstractText | Satellite cells play a central role in mediating the growth and regeneration of skeletal muscle. However, whether satellite cells are stem cells, committed progenitors, or dedifferentiated myoblasts has remained unclear. Using Myf5-Cre and ROSA26-YFP Cre-reporter alleles, we observed that in vivo 10% of sublaminar Pax7-expressing satellite cells have never expressed Myf5. Moreover, we found that Pax7(+)/Myf5(-) satellite cells gave rise to Pax7(+)/Myf5(+) satellite cells through apical-basal oriented divisions that asymmetrically generated a basal Pax7(+)/Myf5(-) and an apical Pax7(+)/Myf5(+) cells. Prospective isolation and transplantation into muscle revealed that whereas Pax7(+)/Myf5(+) cells exhibited precocious differentiation, Pax7(+)/Myf5(-) cells extensively contributed to the satellite cell reservoir throughout the injected muscle. Therefore, we conclude that satellite cells are a heterogeneous population composed of stem cells and committed progenitors. These results provide critical insights into satellite cell biology and open new avenues for therapeutic treatment of neuromuscular diseases. |
