| First Author | Boland B | Year | 1993 |
| Journal | J Muscle Res Cell Motil | Volume | 14 |
| Issue | 1 | Pages | 133-9 |
| PubMed ID | 8478423 | Mgi Jnum | J:13345 |
| Mgi Id | MGI:61546 | Doi | 10.1007/BF00132187 |
| Citation | Boland B, et al. (1993) Lack of dystrophin but normal calcium homeostasis in smooth muscle from dystrophic mdx mice. J Muscle Res Cell Motil 14(1):133-9 |
| abstractText | The free cytosolic Ca2+ concentration ([Ca2+]i) in the dystrophin-lacking smooth muscle from mdx mice was studied to gain new insights into the relation between dystrophin and cytoplasmic Ca2+ homeostasis, which was reported to be impaired in the mdx skeletal muscle. We observed that [Ca2+]i, as measured with the fluorescent Ca2+ indicator fura-2, was not elevated in resting smooth muscle of the vas deferens from mdx mice, in comparison with control C57 mice. Changes of the external Ca2+ concentration evoked similar changes of [Ca2+]i in mdx and control vas deferens. During contraction, cytosolic Ca2+ transients were identical, both in amplitude and in kinetics, whether or not dystrophin was present. Stretches evoked similar Ca2+ increases in muscles from both strains. Intracellular Ca2+ homeostasis appears to be unimpaired in mdx smooth muscle. Thus, the lack of dystrophin per se does not automatically induce a perturbation of Ca metabolism in muscle cells. |
