| First Author | Li R | Year | 2012 |
| Journal | PLoS One | Volume | 7 |
| Issue | 5 | Pages | e37195 |
| PubMed ID | 22606350 | Mgi Jnum | J:187242 |
| Mgi Id | MGI:5435972 | Doi | 10.1371/journal.pone.0037195 |
| Citation | Li R, et al. (2012) Vitronectin increases vascular permeability by promoting VE-cadherin internalization at cell junctions. PLoS One 7(5):e37195 |
| abstractText | BACKGROUND: Cross-talk between integrins and cadherins regulates cell function. We tested the hypothesis that vitronectin (VN), a multi-functional adhesion molecule present in the extracellular matrix and plasma, regulates vascular permeability via effects on VE-cadherin, a critical regulator of endothelial cell (EC) adhesion. METHODOLOGY/PRINCIPAL FINDINGS: Addition of multimeric VN (mult VN) significantly increased VE-cadherin internalization in human umbilical vein EC (HUVEC) monolayers. This effect was blocked by the anti-alpha(V)beta(3) antibody, pharmacological inhibition and knockdown of Src kinase. In contrast to mult VN, monomeric VN did not trigger VE-cadherin internalization. In a modified Miles assay, VN deficiency impaired vascular endothelial growth factor-induced permeability. Furthermore, ischemia-induced enhancement of vascular permeability, expressed as the ratio of FITC-dextran leakage from the circulation into the ischemic and non-ischemic hindlimb muscle, was significantly greater in the WT mice than in the Vn(-/-) mice. Similarly, ischemia-mediated macrophage infiltration was significantly reduced in the Vn(-/-) mice vs. the WT controls. We evaluated changes in the multimerization of VN in ischemic tissue in a mouse hindlimb ischemia model. VN plays a previously unrecognized role in regulating endothelial permeability via conformational- and integrin-dependent effects on VE-cadherin trafficking. CONCLUSION/SIGNIFICANCE: These results have important implications for the regulation of endothelial function and angiogenesis by VN under normal and pathological conditions. |
