| First Author | de Villiers WJ | Year | 1998 |
| Journal | Arterioscler Thromb Vasc Biol | Volume | 18 |
| Issue | 4 | Pages | 631-40 |
| PubMed ID | 9555870 | Mgi Jnum | J:47535 |
| Mgi Id | MGI:1203748 | Doi | 10.1161/01.atv.18.4.631 |
| Citation | de Villiers WJ, et al. (1998) Macrophage phenotype in mice deficient in both macrophage-colony-stimulating factor (op) and apolipoprotein E. Arterioscler Thromb Vasc Biol 18(4):631-40 |
| abstractText | Mice deficient in both macrophage-colony-stimulating factor (M-CSF, op) and apolipoprotein E (apoE) have elevated cholesterol levels but are protected from atherosclerosis, To assess the contribution of macrophage (M phi) phenotypic heterogeneity and scavenger receptor (SR-A) expression to this seeming paradox, we characterized the M phi phenotype by immunohistochemistry in these animals. Lesion size was determined in animals fed a chow or Western-type diet, and lipoprotein clearance studies were performed in vivo. Op0/E0 mice have fourfold smaller aortic root lesions than op2/E0 animals despite 2.5-fold higher total plasma cholesterol levels. M phi s in atherosclerotic lesions of op2/E0 mice constitute a predominantly recruited and M-CSF-dependent population. In addition, M phi s in different locations in plaques show phenotypic heterogeneity. SR-A expression in op0/E0 mice is reduced in proportion to the decrease in M phi numbers, and M-CSF is thus not an essential requirement for SR-A expression in vivo. M-CSF-deficient mice degrade injected AcLDL, showing an adequate level of SR-A activity present in vivo. In contrast, beta-VLDL clearance in op0/E0 mice is decreased, implicating monocytes/M phi s in its catabolism. There is prominent lipid accumulation in op2/ E0 Kupffer cells and hepatocytes but not in M-CSF- independent Kupffer M phi s from op0/E0 mice. SR-A, while abundantly expressed on both Kupffer cells and sinusoidal endothelial cells in op2/E0 mice, remains mainly on sinusoidal endothelial cells in op0/E0 mice. This may explain preservation of SR-A activity in these animals. Our findings clearly illustrate the importance of both M- CSF and M-CSF-dependent monocytes/M phi s in maintaining cholesterol homeostasis and in atherogenesis. |
