| First Author | Dumont FJ | Year | 1983 |
| Journal | Eur J Immunol | Volume | 13 |
| Issue | 6 | Pages | 455-9 |
| PubMed ID | 6602708 | Mgi Jnum | J:7094 |
| Mgi Id | MGI:55565 | Doi | 10.1002/eji.1830130605 |
| Citation | Dumont FJ, et al. (1983) A monoclonal antibody (100C5) to the Lyt-2-T cell population expanding in MRL/Mp-lpr/lpr mice detects a surface antigen normally expressed on Lyt-2+ cells and B cells. Eur J Immunol 13(6):455-9 |
| abstractText | MRL/Mp-lpr/lpr (MRL-lpr) mice develop a generalized lymphadenopathy reflecting the expansion of a Lyt-2- T cell population. The present report describes the pattern of reactivity of a xenogeneic monoclonal antibody (mAb 100C5) directed against this T cell population. By single- and two-color flow cytofluorometry analysis this mAb was found to stain brightly 80-90% of T cells in enlarged MRL-lpr lymph nodes. In contrast lymph node T cells from congenic MRL-MP-+/+ (MRL-+) mice were either unstained (70%) or weakly stained (30%), these latter cells being mostly Lyt-2+. Unexpectedly, all lymph node B cells from MRL-+ or MRL-lpr mice were as strongly 100C5+ as MRL-lpr T cells. Similar observations were made in C57BL/6-lpr/lpr and C57BL/6-+/+ mice. Molecular weight determination suggested that the 100C5 mAb binds to the same molecule (Mr = 220 000) on MRL-lpr T cells and normal B cells. |
