| First Author | De Sevilla Müller LP | Year | 2013 |
| Journal | J Comp Neurol | Volume | 521 |
| Issue | 11 | Pages | 2486-501 |
| PubMed ID | 23296739 | Mgi Jnum | J:315106 |
| Mgi Id | MGI:6765024 | Doi | 10.1002/cne.23294 |
| Citation | De Sevilla Muller LP, et al. (2013) Expression of voltage-gated calcium channel alpha(2)delta(4) subunits in the mouse and rat retina. J Comp Neurol 521(11):2486-501 |
| abstractText | High-voltage activated Ca channels participate in multiple cellular functions, including transmitter release, excitation, and gene transcription. Ca channels are heteromeric proteins consisting of a pore-forming alpha(1) subunit and auxiliary alpha(2)delta and beta subunits. Although there are reports of alpha(2)delta(4) subunit mRNA in the mouse retina and localization of the alpha(2)delta(4) subunit immunoreactivity to salamander photoreceptor terminals, there is a limited overall understanding of its expression and localization in the retina. alpha(2)delta(4) subunit expression and distribution in the mouse and rat retina were evaluated by using reverse transcriptase polymerase chain reaction, western blot, and immunohistochemistry with specific primers and a well-characterized antibody to the alpha(2)delta(4) subunit. alpha(2)delta(4) subunit mRNA and protein are present in mouse and rat retina, brain, and liver homogenates. Immunostaining for the alpha(2)delta(4) subunit is mainly localized to Muller cell processes and endfeet, photoreceptor terminals, and photoreceptor outer segments. This subunit is also expressed in a few displaced ganglion cells and bipolar cell dendrites. These findings suggest that the alpha(2)delta(4) subunit participates in the modulation of L-type Ca(2+) current regulating neurotransmitter release from photoreceptor terminals and Ca(2+)-dependent signaling pathways in bipolar and Muller cells. |
