| First Author | Niederkorn JY | Year | 2006 |
| Journal | Am J Transplant | Volume | 6 |
| Issue | 4 | Pages | 705-13 |
| PubMed ID | 16539627 | Mgi Jnum | J:135761 |
| Mgi Id | MGI:3794409 | Doi | 10.1111/j.1600-6143.2006.01237.x |
| Citation | Niederkorn JY, et al. (2006) Differential roles of CD8+ and CD8- T lymphocytes in corneal allograft rejection in 'high-risk' hosts. Am J Transplant 6(4):705-13 |
| abstractText | We examined the role of perforin and FasL in corneal allograft rejection mediated by CD8+ and CD8 T cells. BALB/c corneas were transplanted orthotopically into vascularized, 'high-risk' graft beds in C57BL/6 mice, perforin knockout mice and FasL-defective gld/gld mice. CD8+ and CD8 T cells were collected following graft rejection and adoptively transferred to SCID mice, which were then challenged with BALB/c corneal allografts. In every case, CD8 T cells could mediate graft rejection when adoptively transferred to SCID mice that received BALB/c corneal allografts. Although CD8+ T cells also mediated graft rejection, the tempo was slower. Moreover, CD8+ T cells collected FasL-defective donors that had rejected corneal allografts, mediated corneal allograft rejection in only 50% of the SCID mice that received the adoptively transferred cells. In some cases, CD8+ T-cell-mediated rejection occurred in the absence of delayed-type hypersensitivity and cytotoxic T-lymphocyte activity, but was associated with CD8+ T-cell-mediated apoptosis of BALB/c corneal cells in vitro. The results demonstrate the redundancy in immune mechanisms of corneal allograft rejection. Either CD8+ or CD8 T cells can produce corneal allograft rejection, however functional FasL is necessary for optimal rejection, even in a high-risk setting. |
