| First Author | Kim J | Year | 2011 |
| Journal | Sci Rep | Volume | 1 |
| Pages | 122 | PubMed ID | 22355639 |
| Mgi Jnum | J:206121 | Mgi Id | MGI:5547909 |
| Doi | 10.1038/srep00122 | Citation | Kim J, et al. (2011) Altered branching patterns of Purkinje cells in mouse model for cortical development disorder. Sci Rep 1:122 |
| abstractText | Disrupted cortical cytoarchitecture in cerebellum is a typical pathology in reeler. Particularly interesting are structural problems at the cellular level: dendritic morphology has important functional implication in signal processing. Here we describe a combinatorial imaging method of synchrotron X-ray microtomography with Golgi staining, which can deliver 3-dimensional(3-D) micro-architectures of Purkinje cell(PC) dendrites, and give access to quantitative information in 3-D geometry. In reeler, we visualized in 3-D geometry the shape alterations of planar PC dendrites (i.e., abnormal 3-D arborization). Despite these alterations, the 3-D quantitative analysis of the branching patterns showed no significant changes of the 77 +/- 8 degrees branch angle, whereas the branch segment length strongly increased with large fluctuations, comparing to control. The 3-D fractal dimension of the PCs decreased from 1.723 to 1.254, indicating a significant reduction of dendritic complexity. This study provides insights into etiologies and further potential treatment options for lissencephaly and various neurodevelopmental disorders. |
