| First Author | Edwards JA | Year | 1975 |
| Journal | Blood | Volume | 46 |
| Issue | 3 | Pages | 381-8 |
| PubMed ID | 807277 | Mgi Jnum | J:5555 |
| Mgi Id | MGI:54032 | Doi | 10.1182/blood.v46.3.381.bloodjournal463381 |
| Citation | Edwards JA, et al. (1975) Red cell iron uptake in hereditary microcytic anemia. Blood 46(3):381-8 |
| abstractText | The iron uptake in vitro of red cells from mice with hereditary microcytic anemia (gene symbol mk) was studied to examine the hypothesis of a generalized impairment of cellular iron uptake in this conidition. Reticulocyte-rich red cells from anemic (mk/mk) and acutely bled normal (+/+) mice were incubated in 59Fe-labeled mouse plasma and the radioiron uptake measured. The 59Fe uptake of the mk/mk and +/+ cells was related in the same way to the reticulocyte concentration, the duration of incubation, and the percentage saturation of the plasma iron-binding capacity. However, under the same conditions, the iron uptake of red cells from normal (+/+) mice was greater than that by red cells from anemic (mk/mk) mice. Furthermore, the cellular loss of radioiron on exposure to EDTA was greater for the mk/mk red cells, although the proportion of the radioiron taken up that was incorporated into heme was the same for mk/mk and +/+ red cells. These results support the hypothesis of a generalized impairment of cellular iron uptake in hereditary microcytic anemia and suggest that there might be a defect in red cell receptor sites for transferrin in this condition. |
