| First Author | Gurung P | Year | 2016 |
| Journal | Proc Natl Acad Sci U S A | Volume | 113 |
| Issue | 16 | Pages | 4452-7 |
| PubMed ID | 27071119 | Mgi Jnum | J:232215 |
| Mgi Id | MGI:5776324 | Doi | 10.1073/pnas.1601636113 |
| Citation | Gurung P, et al. (2016) NLRP3 inflammasome plays a redundant role with caspase 8 to promote IL-1beta-mediated osteomyelitis. Proc Natl Acad Sci U S A 113(16):4452-7 |
| abstractText | Missense mutation in the proline-serine-threonine phosphatase-interacting protein 2 (Pstpip2) gene results in the development of spontaneous chronic bone disease characterized by bone deformity and inflammation that is reminiscent of patients with chronic multifocal osteomyelitis (cmo). Interestingly, this disease is specifically mediated by IL-1beta but not IL-1alpha. The precise molecular pathways that promote pathogenic IL-1beta production inPstpip2(cmo)mice remain unidentified. Furthermore, how IL-1beta provokes inflammatory bone disease inPstpip2(cmo)mice is not known. Here, we demonstrate that double deficiency of Nod like receptor family, pyrin domain containing 3 (NLRP3) and caspase 8 inPstpip2(cmo)mice provides similar protection as observed in caspase-1 and caspase-8-deficientPstpip2(cmo)mice, demonstrating redundant roles for the NLRP3 inflammasome and caspase 8 in provoking osteomyelitic disease inPstpip2(cmo)mice. Consistently, immunofluorescence studies exhibited distinct caspase-1 and caspase-8 puncta in diseasedPtpn6(spin)neutrophils. Data from our chimera studies demonstrated that IL-1beta produced by hematopoietic cells is sensed by the radioresistant compartment to promote bone disease. Furthermore, our results showed that the IL-1beta signaling is unidirectional and feedback signaling of IL-1beta onto the hematopoietic compartment is not important for disease induction. In conclusion, our studies have uncovered the combined actions of the NLRP3 inflammasome and caspase 8 leading to IL-1beta maturation and the directionality of IL-1beta in driving disease inPstpip2(cmo)mice. |
