| First Author | de Bruin RG | Year | 2016 |
| Journal | Sci Rep | Volume | 6 |
| Pages | 21643 | PubMed ID | 26905650 |
| Mgi Jnum | J:253763 | Mgi Id | MGI:6102615 |
| Doi | 10.1038/srep21643 | Citation | de Bruin RG, et al. (2016) The RNA-binding protein quaking maintains endothelial barrier function and affects VE-cadherin and beta-catenin protein expression. Sci Rep 6:21643 |
| abstractText | Proper regulation of endothelial cell-cell contacts is essential for physiological functioning of the endothelium. Interendothelial junctions are actively involved in the control of vascular leakage, leukocyte diapedesis, and the initiation and progression of angiogenesis. We found that the RNA-binding protein quaking is highly expressed by endothelial cells, and that its expression was augmented by prolonged culture under laminar flow and the transcription factor KLF2 binding to the promoter. Moreover, we demonstrated that quaking directly binds to the mRNA of VE-cadherin and beta-catenin and can induce mRNA translation mediated by the 3''UTR of these genes. Reduced quaking levels attenuated VE-cadherin and beta-catenin expression and endothelial barrier function in vitro and resulted in increased bradykinin-induced vascular leakage in vivo. Taken together, we report that quaking is essential in maintaining endothelial barrier function. Our results provide novel insight into the importance of post-transcriptional regulation in controlling vascular integrity. |
